Regulatory

Part:BBa_K4115016:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-23)


PcsiE, a starvation induced promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcsiE is controlled by two factors that are related to starvation condition.


Source

We amplified this part from E.coli DH5-slpha genome through PCR.

References

[1] Marschall, C., and Hengge-Aronis, R. (1995). Regulatory characteristics and promoter analysis of csiE, a stationary phase-inducible gene under the control of sigma S and the cAMP-CRP complex in Escherichia coli. Mol Microbiol 18, 175-184. 10.1111/j.1365-2958.1995.mmi_18010175.x.
[2] Brognaux, A., Thonart, P., Delvigne, F., Neubauer, P., Twizere, J.C., Francis, F., and Gorret, N. (2013). Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: design of milliliter scale-down bioreactors. Biotechnol Prog 29, 48-59. 10.1002/btpr.1660.